The International Rice Genome Sequencing Project (IRGSP) 
Historically, PGIR was established as a high-throughput sequencing facility of medium capacity to participate in an international effort to sequence the rice genome. PGIR had been assigned the middle portion of chromosome 10 from 29.8 to 41.8 cM. We have completed the sequence of this region, the minimum-tiling path of which consists of 22 BAC clones that add up to approximately 2.9 Mb (3,131,203 bp including overlap). The upstream region of chromosome 10 has been sequenced by a consortium of Clemson University, Cold Spring Harbor Laboratory, and Washington University, while sequencing of the downstream region has been undertaken by TIGR. The entire chromosome has a length of 22.4 Mb and predicted to have 3,471 genes.

PGIR has also participated in the sequencing of chromosome 11, which had been assigned to the US and India. We have sequenced a total of 22 BAC clones up to phase 2 level from this chromosome as well although only to phase 2 level, which add up to a total of 2,919 kb (excluding overlap). The University of Arizona (member of IRGSP) will finish these clones to phase 3 level with USDA support. The estimated length of chromosome 11 is 30 Mb.

During the course of sequencing clones from chromosomes 10 and 11, we also sequenced one clone from chromosome 12 and two smaller clones from the indica variety Teqing that have also been deposited to GenBank.



Recent Publication:
  • International Rice Genome Sequencing Project (2005) The map-based sequence of the rice genome. Nature 436: 793-800.
  • The Rice Chromosomes 11 and 12 Sequencing Consortia (2005) The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications. BMC Biol. 3: 20.
  • Rice Chromosome 10 Sequencing Consortium (2003). In-depth view of structure, activity, and evolution of rice chromosome 10. Science 300, 1566-1569.

Poster Presentation:

Our portion of the project has been supported through the Rutgers Reinvest Program.