The International Rice Genome Sequencing Project (IRGSP)
Historically, PGIR was established as a high-throughput sequencing facility of medium capacity to participate in an international effort to sequence the rice genome. PGIR had been assigned the middle portion of chromosome 10 from 29.8 to 41.8 cM. We have completed the sequence of this region, the minimum-tiling path of which consists of 22 BAC clones that add up to approximately 2.9 Mb (3,131,203 bp including overlaps). A consortium of Clemson University, Cold Spring Harbor Laboratory, and Washington University has sequenced the upstream region of chromosome 10, whereas TIGR has undertaken sequencing of the downstream region. The entire chromosome has a length of 22.4 Mb and predicted to have 3,471 genes. PGIR has also participated in the sequencing of chromosome 11, which had been assigned to the US and India. We have sequenced a total of 22 BAC clones up to phase-2 level from this chromosome, which add up to a total of 2,919 kb (excluding overlaps). The University of Arizona (member of IRGSP) has finished these clones to phase-3 level with USDA support. The estimated length of chromosome 11 is 30MB.